Thursday, January 21, 2016

Candy Electrophoresis Lab

1.)There were no colors that did not match the reference bands
2.)Fast Green FCR and Red 40 are most likely to move like the colors we saw in the lab because they have a similar chemical structure to the reference dyes.
3.) Dog food companies put artificial colors in dog food to make it look more appealing. Humans are more likely to buy dog food that looks nice and that has bold colors. They think that this food will be better for their dogs. This is ironic, because many artificial colors have unknown side affects on dogs and humans.
4.) Most Commonly Used Food Colors with Natural Alternatives


FD&C Dye
Color
Natural Alternative
Source for Natural Alternative
Blue 1
Blue
Spirulina Extract
Cyanobacteria
Blue 2
Blue
blueberry juice
blueberries
Green 3
Green
Spinach juice
Spinach
Red 3
Red
Beet Juice
Beets
Red 40
Red
Purple Sweet Potato juice
Purple Sweet Potato
Yellow 5
Yellow
turmeric
turmeric
Yellow 6
Yellow
paprika
paprika
Citrus Red 2
Orange
Beta-Carotene
Carrots
Artificial colors are used rather than natural alternatives because they are cheaper and easier to obtain.
5.) The 2 factors that control the distance that the colored dye travels are the size of the DNA in the dye and
6.) The fact that DNA is charged negatively propels it. When electricity is turned on, DNA will go towards the positive side.
7.) The fact that the longer the DNA is, the slower it moves, separates DNA by size.
8.) If DNA with a weight of 600, 1000, 2000, and 5000 daltons were put into a gel electrophoresis, I would expect that the 600 would travel furthest, with 100 not to far behind. 2000 would be further back, and 5000 would be in last.
Our Gel

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